Part:BBa_K4587001:Design

L4440 vector

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 65
  Illegal EcoRI site found at 161
  Illegal XbaI site found at 95
  Illegal SpeI site found at 101
  Illegal PstI site found at 155
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 65
  Illegal EcoRI site found at 161
  Illegal NheI site found at 125
  Illegal SpeI site found at 101
  Illegal PstI site found at 155
  Illegal NotI site found at 87
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 65
  Illegal EcoRI site found at 161
  Illegal BglII site found at 47
  Illegal BamHI site found at 107
  Illegal BamHI site found at 143
  Illegal XhoI site found at 194
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 65
  Illegal EcoRI site found at 161
  Illegal XbaI site found at 95
  Illegal SpeI site found at 101
  Illegal PstI site found at 155
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 65
  Illegal EcoRI site found at 161
  Illegal XbaI site found at 95
  Illegal SpeI site found at 101
  Illegal PstI site found at 155
  Illegal NgoMIV site found at 534
  Illegal AgeI site found at 113
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 1710
  Illegal BsaI.rc site found at 38
  

Design Notes

There are various L4440 vectors with different antibiotic markers, therefore, it is important to choose the one with a stable antibiotic to guarantee the efficiency of the following selection of desired bacteria.

Source

https://novoprolabs.com/vector/Vgezdoobu